Device

Part:BBa_K2272020:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-31)


pBAD-HAO-Cyt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1556
    Illegal XhoI site found at 3120
    Illegal XhoI site found at 5329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 2049
    Illegal NgoMIV site found at 2053
    Illegal NgoMIV site found at 3821
    Illegal NgoMIV site found at 4211
    Illegal NgoMIV site found at 4478
    Illegal NgoMIV site found at 4526
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476
    Illegal BsaI.rc site found at 1524
    Illegal BsaI.rc site found at 2091
    Illegal BsaI.rc site found at 4251
    Illegal BsaI.rc site found at 5928
    Illegal BsaI.rc site found at 6084
    Illegal SapI site found at 961


Design Notes

pbad


Source

BBa_K2272002, BBa_K2272003

References